Hydrogen peroxide temperature celery goggles and an apron or lab coat to protect our eyes and clothes as we are using enzymes and hydrogen peroxide we need to be extra careful, ensuring they don't come into contact with our eyes, skin or clothes catalyse is an enzyme found in all living cells. In this catalase and hydrogen peroxide experiment, we will discover how enzymes act as catalysts by causing chemical reactions to occur more quickly within living things using a potato and hydrogen peroxide, we can observe how enzymes like catalase work to perform decomposition, or the breaking down, of other substances catalase works to speed up the decomposition of hydrogen peroxide into oxygen and water.
Hydrogen peroxide is used to detect the presence of enzymes in liver, potato and celery, which catalyse the decomposition of hydrogen peroxide, by detecting the presence of the oxygen gas formed.
Place a small amount of hydrogen peroxide solution in a container add a few drops of washing up liquid and then some potato (or celery) pieces compare the speed of bubble formation when yeast is added. Take 2 stalks of celery and add to 100 ml of water use a blender to mix the water and celery until no large lumps remain filter the mixture through filter paper in a funnel and keep the liquid part (filtrate) in a beaker.
Note the units for measuring the concentration of hydrogen peroxide – these are not si units 10 vol hydrogen peroxide will produce 10 cm 3 of oxygen from every cm 3 that decomposes note 1 ) in this procedure, 2 cm 3 of 10 vol hydrogen peroxide will release 20 cm 3 of oxygen if the reaction goes to completion 2 cm 3 of liquid are added to the flask each time. Furthermore, due to the exothermic nature of the reaction, dilution will diminish any temperature increase, thereby making the experiment near-isothermal the scope of this kinetic experiment is to gain insight in the catalytic performance of yeast as a catalytic microfactory for the conversion of hydrogen peroxide to water and oxygen. Hydrogen peroxide (h 2 o 2) is commonly used as a hair bleach it also has an interesting property in that it decomposes over time producing oxygen and water 2h 2 o 2 2h 2 o + o 2 the oxygen can be seen here as it is a gas which blows bubbles in the soapy water. Celery and (b) animal liver, on the rate of breakdown of hydrogen peroxide catalase causes this oxygen will make bubbles in washingup liquid so we can measure the amount of bubbles to see how well the enzyme catalase worked.
Potato or celery yeast washing up liquid hydrogen peroxide solution (3%, 6% or 9% for hair bleaching is suitable) container method: place a small amount of hydrogen peroxide solution in a container add a few drops of washing up liquid and then some potato (or celery) pieces compare the speed of bubble formation when yeast is added.
Hydrogen peroxide (h 2 o 2) detergent solution (this is best watered down or it just sits on top of the h 2 o 2) potassium iodide (ki) vegetable pieces (potato and celery are really good) 2 x 100 ml measuring cylinders 2 x plastic pipettes spatula tray water what to do 1 pipette 10 ml of hydrogen peroxide into each measuring cylinder on the tray 2 add a few drops of detergent solution to each measuring cylinder 3. A measure 25 cm 3 of hydrogen peroxide solution into each of three conical flasks b at the same time, add a small piece of liver to the first flask, a small piece of potato to the second flask, and a small piece of celery to the third flask c hold a glowing splint in the neck of each flask.