Differentiating gram-negative and gram-positive bacteria the gram-stain is the single most common and cost effective staining technique used in identifying bacterial organisms the method was originally devised in 1884 by a danish bacteriologist, hans christian gram. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives the procedure is based on the ability of microorganisms to retain color of the stains used during the gram stain reaction.
The gram stain procedure enables bacteria to retain color of the stains, based on the differences in the chemical and physical properties of the cell wall 1 gram positive bacteria : stain dark purple due to retaining the primary dye called crystal violet in the cell wall. A student creates a gram stain on a bacterial specimen that has a mix of gram-negative and gram-positive organisms but accidentally forgets the decolorizer step.
Mastering in micro chapter 3 study guide by niani_conerly includes 48 questions covering vocabulary, terms and more which step in the gram stain is the critical step in differentiating gram-positive cells from gram-negative cells a student creates a gram stain on a bacterial specimen that has a mix of gram-negative and gram-positive. Bacteria are often classified into gram-positive and gram-negative strains by their visual staining properties using crystal violet (cv), a triarylmethane dye here we show that bioorthogonal modification of crystal violet with transcyclooctene can be used to render gram-positive bacteria magnetic. Amanda lab report 1 microbiology laboratory title – differentiating organisms using the gram stain introduction the experiment conducted was based upon the known attributes of two different groups of bacteria, those that are gram positive, and those that are gram negative. Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents the gram stain procedure distinguishes between gram positive and gram negative groups by coloring these cells red or violet.
Using a specific staining procedure, it is possible to differentiate the two types under a microscope the gram stain method of differentiation is possible because of differences in the cell membrane between the two categories of bacteria.
Besides gram's stain, there is a wide range of staining methods available the procedures for these other methods follow quite closely those of gram's stain by using appropriate dyes, different parts of the cell structure such as capsules, flagella, granules, or spores can be stained.
Gram stain or gram staining, also called gram's method, is a method of staining used to distinguish and classify bacterial species into two large groups (gram-positive and gram-negative) the name comes from the danish bacteriologist hans christian gram , who developed the technique.